In Situ Hybridization in Electron Microscopy (Methods in by Gerard Morel,Annie Cavalier,Lynda Williams

By Gerard Morel,Annie Cavalier,Lynda Williams

In situ hybridization is a method that permits for the visualization of particular DNA and RNA sequences in person cells, and is an extremely vital technique for learning nucleic acids in heterogeneous phone populations. in situ Hybridization in Electron Microscopy stories the 3 major equipment constructed for the ultrastructural visualization of genes:
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization sooner than embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each one strategy, different phases are defined intimately: the practise of tissue, pretreatment, hybridization, and visualization of the hybridization items.

The ebook combines idea and perform, beginning with the elemental rules, then breaking down the experimental procedure into successive steps illustrated via a number of diagrams, distinct protocols, and tables. this can be all performed in a structure that makes use of parallel columns to show worthwhile reviews subsequent to the speculation and sensible info along each one level of the protocol. also, the precis tables give you the standards for selecting the probe variety and strategy, and an in depth index aids within the look for details. in situ Hybridization In Electron Microscopy is a necessary better half for using those equipment on the electron microscopic level.

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